BAIT
FEN1
FEN-1, MF1, RAD2
flap structure-specific endonuclease 1
GO Process (14)
GO Function (10)
GO Component (6)
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [IDA, IMP]
- DNA catabolic process, exonucleolytic [TAS]
- DNA repair [TAS]
- DNA replication [TAS]
- DNA replication, removal of RNA primer [IDA]
- DNA strand elongation involved in DNA replication [TAS]
- RNA phosphodiester bond hydrolysis, endonucleolytic [IDA]
- UV protection [TAS]
- base-excision repair [TAS]
- double-strand break repair [TAS]
- mitotic cell cycle [TAS]
- telomere maintenance [TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
Gene Ontology Molecular Function- 5'-3' exonuclease activity [IDA]
- 5'-flap endonuclease activity [IDA, IMP]
- DNA binding [IMP]
- RNA-DNA hybrid ribonuclease activity [IDA]
- damaged DNA binding [TAS]
- double-stranded DNA binding [TAS]
- double-stranded DNA exodeoxyribonuclease activity [TAS]
- endonuclease activity [TAS]
- exonuclease activity [TAS]
- protein binding [IPI]
- 5'-3' exonuclease activity [IDA]
- 5'-flap endonuclease activity [IDA, IMP]
- DNA binding [IMP]
- RNA-DNA hybrid ribonuclease activity [IDA]
- damaged DNA binding [TAS]
- double-stranded DNA binding [TAS]
- double-stranded DNA exodeoxyribonuclease activity [TAS]
- endonuclease activity [TAS]
- exonuclease activity [TAS]
- protein binding [IPI]
Gene Ontology Cellular Component
Homo sapiens
PREY
RAB14
FBP, RAB-14, RP11-165P4.4
RAB14, member RAS oncogene family
GO Process (12)
GO Function (5)
GO Component (19)
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- Golgi to endosome transport [ISS, TAS]
- Rab protein signal transduction [IBA]
- embryo development [ISS]
- endocytic recycling [IDA]
- fibroblast growth factor receptor signaling pathway [ISS]
- intracellular protein transport [IBA]
- intracellular transport [NAS]
- membrane organization [TAS]
- phagolysosome assembly involved in apoptotic cell clearance [IBA]
- regulation of protein localization [IDA]
- vesicle-mediated transport [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IBA]
- Golgi stack [ISS]
- cytoplasmic vesicle membrane [TAS]
- cytosol [ISS]
- early endosome [IBA, ISS]
- extracellular vesicular exosome [IDA]
- intracellular [IDA]
- intracellular membrane-bounded organelle [IDA]
- late endosome [ISS]
- lysosomal membrane [IDA]
- lysosome [ISS]
- nuclear outer membrane-endoplasmic reticulum membrane network [ISS]
- perinuclear region of cytoplasm [ISS]
- phagocytic vesicle [IDA]
- plasma membrane [ISS]
- primary cilium [IDA]
- recycling endosome [IDA]
- rough endoplasmic reticulum [ISS]
- trans-Golgi network transport vesicle [ISS]
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MDA231 cell line (score 0.675)
Curated By
- BioGRID