BAIT
NSF
SKD2
N-ethylmaleimide-sensitive factor
GO Process (10)
GO Function (7)
GO Component (8)
Gene Ontology Biological Process
- ATP catabolic process [ISS]
- Golgi to plasma membrane protein transport [IBA]
- Golgi vesicle docking [IBA]
- exocytosis [TAS]
- intra-Golgi vesicle-mediated transport [IBA]
- plasma membrane fusion [TAS]
- positive regulation of receptor recycling [IDA]
- regulation of exocytosis [ISS]
- synaptic transmission [TAS]
- vesicle-mediated transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
LRRC16A
CARMIL, CARMIL1, CARMIL1a, LRRC16, dJ501N12.1, dJ501N12.5
leucine rich repeat containing 16A
GO Process (13)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
- actin filament organization [NAS]
- barbed-end actin filament uncapping [ISS]
- blood coagulation [TAS]
- cell migration [IMP]
- lamellipodium assembly [IMP]
- negative regulation of barbed-end actin filament capping [ISS]
- positive regulation of actin filament polymerization [ISS]
- positive regulation of cell migration [IMP]
- positive regulation of lamellipodium organization [IDA]
- positive regulation of stress fiber assembly [IMP]
- positive regulation of substrate adhesion-dependent cell spreading [IMP]
- ruffle organization [IMP]
- urate metabolic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF7 cell line (score 0.658)
Curated By
- BioGRID