BAIT
TUFM
COXPD4, EF-TuMT, EFTU, P43
Tu translation elongation factor, mitochondrial
GO Process (1)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
MYO6
DFNA22, DFNB37, RP3-472A9.1
myosin VI
GO Process (9)
GO Function (7)
GO Component (19)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator [IDA]
- actin filament-based movement [ISS, NAS]
- endocytosis [IMP, ISS]
- intracellular protein transport [ISS]
- membrane organization [TAS]
- metabolic process [ISS, NAS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of secretion [IMP]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- DNA-directed RNA polymerase II, holoenzyme [IDA]
- Golgi apparatus [IDA]
- cell cortex [ISS]
- clathrin-coated endocytic vesicle [IDA]
- cytoplasm [IDA, ISS]
- cytoplasmic membrane-bounded vesicle [IDA]
- cytosol [TAS]
- endocytic vesicle [ISS]
- extracellular vesicular exosome [IDA]
- filamentous actin [IDA, ISS]
- lysosomal membrane [TAS]
- membrane [IDA]
- nuclear membrane [IDA]
- nucleoplasm [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA, ISS]
- plasma membrane [TAS]
- ruffle [IDA]
- unconventional myosin complex [TAS]
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.66)
Curated By
- BioGRID