BAIT
CTCF
MRD21
CCCTC-binding factor (zinc finger protein)
GO Process (8)
GO Function (9)
GO Component (5)
Gene Ontology Biological Process
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- nucleosome positioning [IDA]
- positive regulation of gene expression [IMP]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- regulation of centromeric sister chromatid cohesion [NAS]
- regulation of gene expression, epigenetic [IMP]
- regulation of molecular function, epigenetic [IMP]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- chromatin insulator sequence binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [TAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- chromatin insulator sequence binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [TAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [TAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
KPNA1
IPOA5, NPI-1, RCH2, SRP1
karyopherin alpha 1 (importin alpha 5)
GO Process (10)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- NLS-bearing protein import into nucleus [TAS]
- apoptotic DNA fragmentation [TAS]
- apoptotic process [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cytokine-mediated signaling pathway [TAS]
- intracellular transport of virus [TAS]
- positive regulation of protein import into nucleus [ISS]
- regulation of DNA recombination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.78)
- MCF7 cell line (score 0.677)
- MDA231 cell line (score 0.68)
Curated By
- BioGRID