Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Involvement of Arabidopsis ROF2 (FKBP65) in thermotolerance.

Meiri D, Tazat K, Cohen-Peer R, Farchi-Pisanty O, Aviezer-Hagai K, Avni A, Breiman A

The ROF2 (FKBP65) is a heat stress protein which belongs to the FK506 Binding Protein (FKBP) family. It is homologous to ROF1 (FKBP62) which was recently shown to be involved in long term acquired thermotolerance by its interaction with HSP90.1 and modulation of the heat shock transcription factor HsfA2. In this study, we have demonstrated that ROF2 participates in long ... [more]

Plant Mol. Biol. Jan. 01, 2010; 72(1);191-203 [Pubmed: 19876748]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ROF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

FK506 binding [IBA, ISS]calmodulin binding [ISS]peptidyl-prolyl cis-trans isomerase activity [IBA, ISS]phosphatidylinositol-3,5-bisphosphate binding [IDA]phosphatidylinositol-3-phosphate binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

ROF2

Gene Ontology Biological Process

Gene Ontology Molecular Function

FK506 binding [IBA, ISS]calmodulin binding [ISS]peptidyl-prolyl cis-trans isomerase activity [IBA, ISS]protein binding [IPI]

Gene Ontology Cellular Component

Two-hybrid

Publication

Involvement of Arabidopsis ROF2 (FKBP65) in thermotolerance.

Throughput

Curated By

FK506 binding [IBA, ISS]
calmodulin binding [ISS]
peptidyl-prolyl cis-trans isomerase activity [IBA, ISS]
phosphatidylinositol-3,5-bisphosphate binding [IDA]
phosphatidylinositol-3-phosphate binding [IDA]
protein binding [IPI]

FK506 binding [IBA, ISS]
calmodulin binding [ISS]
peptidyl-prolyl cis-trans isomerase activity [IBA, ISS]
protein binding [IPI]