EIF2B2
Gene Ontology Biological Process
- L-methionine biosynthetic process from methylthioadenosine [IBA]
- cellular protein metabolic process [TAS]
- cellular response to stimulus [IDA]
- central nervous system development [IMP]
- gene expression [TAS]
- myelination [IMP]
- oligodendrocyte development [IMP]
- ovarian follicle development [IMP]
- positive regulation of GTPase activity [IDA, IMP]
- regulation of translational initiation [IBA, TAS]
- response to glucose [ISS]
- response to heat [ISS, TAS]
- response to peptide hormone [ISS]
- translation [TAS]
- translational initiation [IDA, TAS]
Gene Ontology Molecular Function
EIF2B5
Gene Ontology Biological Process
- astrocyte development [IMP]
- astrocyte differentiation [IMP]
- cellular protein metabolic process [TAS]
- cellular response to drug [IDA]
- gene expression [TAS]
- myelination [IMP]
- negative regulation of translational initiation in response to stress [ISS]
- oligodendrocyte development [IMP]
- ovarian follicle development [IMP]
- positive regulation of GTPase activity [IDA, IMP, ISS]
- positive regulation of translational initiation [ISS]
- response to endoplasmic reticulum stress [IMP]
- response to glucose [ISS]
- response to heat [IMP, ISS, TAS]
- response to peptide hormone [ISS]
- translation [TAS]
- translational initiation [IDA, TAS]
Gene Ontology Molecular Function
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.755)
- MCF7 cell line (score 0.71)
- MDA231 cell line (score 0.721)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EIF2B5 EIF2B2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| EIF2B2 EIF2B5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2222162 | |
| EIF2B2 EIF2B5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3057204 | |
| EIF2B5 EIF2B2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3193912 | |
| EIF2B2 EIF2B5 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.986 | BioGRID | 741809 | |
| EIF2B5 EIF2B2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 1 | BioGRID | 1261489 | |
| EIF2B2 EIF2B5 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| EIF2B5 EIF2B2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID