LIG4
Gene Ontology Biological Process
- DNA ligation [IDA]
- DNA ligation involved in DNA recombination [ISS]
- DNA ligation involved in DNA repair [IDA, ISS]
- DNA repair [TAS]
- T cell differentiation in thymus [ISS]
- T cell receptor V(D)J recombination [ISS]
- V(D)J recombination [IDA]
- cell proliferation [ISS]
- central nervous system development [ISS]
- chromosome organization [ISS]
- double-strand break repair [IDA, ISS, TAS]
- double-strand break repair via nonhomologous end joining [IDA, IMP, TAS]
- establishment of integrated proviral latency [TAS]
- in utero embryonic development [ISS]
- isotype switching [ISS]
- lagging strand elongation [IBA]
- negative regulation of neuron apoptotic process [ISS]
- neuron apoptotic process [ISS]
- nucleotide-excision repair, DNA gap filling [IDA]
- positive regulation of fibroblast proliferation [ISS]
- positive regulation of neurogenesis [ISS]
- pro-B cell differentiation [ISS]
- response to X-ray [IMP]
- response to gamma radiation [ISS]
- single strand break repair [IDA]
- somatic stem cell maintenance [ISS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PRKDC
Gene Ontology Biological Process
- DNA repair [TAS]
- cellular protein modification process [TAS]
- cellular response to insulin stimulus [IMP]
- double-strand break repair [TAS]
- double-strand break repair via homologous recombination [IBA]
- double-strand break repair via nonhomologous end joining [TAS]
- innate immune response [TAS]
- negative regulation of protein phosphorylation [ISS]
- peptidyl-serine phosphorylation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of type I interferon production [TAS]
- regulation of circadian rhythm [ISS]
- signal transduction involved in mitotic G1 DNA damage checkpoint [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.705)
- MCF7 cell line (score 0.715)
- MDA231 cell line (score 0.667)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| LIG4 PRKDC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PRKDC LIG4 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 823074 | |
| PRKDC LIG4 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | 245129 |
Curated By
- BioGRID