BLM
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA double-strand break processing [IDA]
- DNA duplex unwinding [IDA, IMP]
- DNA recombination [NAS]
- DNA repair [NAS]
- DNA strand renaturation [IDA]
- cellular response to DNA damage stimulus [IDA, IMP]
- cellular response to camptothecin [IDA]
- cellular response to hydroxyurea [IDA]
- cellular response to ionizing radiation [IDA]
- double-strand break repair via homologous recombination [NAS]
- mitotic G2 DNA damage checkpoint [IDA]
- negative regulation of DNA recombination [IMP]
- negative regulation of cell division [IMP]
- positive regulation of transcription, DNA-templated [IDA]
- protein oligomerization [IDA]
- regulation of cyclin-dependent protein serine/threonine kinase activity [IMP]
- replication fork processing [IDA]
- replication fork protection [NAS]
- response to X-ray [IDA]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATP-dependent DNA helicase activity [IDA, IMP]
- ATP-dependent helicase activity [IDA]
- ATPase activity [IDA]
- G-quadruplex DNA binding [IDA]
- annealing helicase activity [IDA]
- bubble DNA binding [IDA]
- four-way junction helicase activity [IDA]
- helicase activity [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- single-stranded DNA binding [IDA]
- ATP binding [IDA]
- ATP-dependent DNA helicase activity [IDA, IMP]
- ATP-dependent helicase activity [IDA]
- ATPase activity [IDA]
- G-quadruplex DNA binding [IDA]
- annealing helicase activity [IDA]
- bubble DNA binding [IDA]
- four-way junction helicase activity [IDA]
- helicase activity [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- single-stranded DNA binding [IDA]
Gene Ontology Cellular Component
RMI1
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleoplasm [IDA]
- nucleus [IDA]
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.714)
- MCF7 cell line (score 0.697)
- MDA231 cell line (score 0.689)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BLM RMI1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3348825 | |
| BLM RMI1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1456192 | |
| BLM RMI1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| BLM RMI1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| RMI1 BLM | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID