ACTB
Gene Ontology Biological Process
- 'de novo' posttranslational protein folding [TAS]
- ATP-dependent chromatin remodeling [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- adherens junction organization [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell junction assembly [TAS]
- cell-cell junction organization [TAS]
- cellular component movement [TAS]
- cellular protein metabolic process [TAS]
- chromatin organization [TAS]
- innate immune response [TAS]
- membrane organization [TAS]
- platelet aggregation [IMP]
- protein folding [TAS]
- retina homeostasis [IEP]
- substantia nigra development [IEP]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- Tat protein binding [IPI]
- kinesin binding [IPI]
- nitric-oxide synthase binding [IPI]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- structural constituent of cytoskeleton [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- Tat protein binding [IPI]
- kinesin binding [IPI]
- nitric-oxide synthase binding [IPI]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- structural constituent of cytoskeleton [TAS]
Gene Ontology Cellular Component
- MLL5-L complex [IDA]
- NuA4 histone acetyltransferase complex [IDA]
- blood microparticle [IDA]
- cytoplasm [TAS]
- cytoplasmic ribonucleoprotein granule [IDA]
- cytoskeleton [TAS]
- cytosol [TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- nuclear chromatin [IDA]
- nucleoplasm [TAS]
- protein complex [IDA]
- ribonucleoprotein complex [IDA]
CARM1
Gene Ontology Biological Process
- cellular lipid metabolic process [TAS]
- histone H3-R17 methylation [ISS]
- histone H3-R2 methylation [IMP]
- histone methylation [IDA, ISS]
- peptidyl-arginine methylation, to asymmetrical-dimethyl arginine [IBA]
- positive regulation of fat cell differentiation [ISS]
- regulation of intracellular estrogen receptor signaling pathway [ISS]
- regulation of transcription, DNA-templated [IBA, ISS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- beta-catenin binding [TAS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-R17 specific) [ISS]
- histone-arginine N-methyltransferase activity [IBA]
- ligand-dependent nuclear receptor transcription coactivator activity [ISS]
- lysine-acetylated histone binding [ISS]
- protein binding [IPI]
- protein methyltransferase activity [ISS]
- protein-arginine N-methyltransferase activity [ISS]
- protein-arginine omega-N asymmetric methyltransferase activity [IBA, ISS]
- transcription coactivator activity [ISS]
- transcription regulatory region DNA binding [ISS]
- beta-catenin binding [TAS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-R17 specific) [ISS]
- histone-arginine N-methyltransferase activity [IBA]
- ligand-dependent nuclear receptor transcription coactivator activity [ISS]
- lysine-acetylated histone binding [ISS]
- protein binding [IPI]
- protein methyltransferase activity [ISS]
- protein-arginine N-methyltransferase activity [ISS]
- protein-arginine omega-N asymmetric methyltransferase activity [IBA, ISS]
- transcription coactivator activity [ISS]
- transcription regulatory region DNA binding [ISS]
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.709)
- MCF7 cell line (score 0.765)
- MDA231 cell line (score 0.763)
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
CARM1 ACTB | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
CARM1 ACTB | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - |
Curated By
- BioGRID