BAIT
AKAP12
AKAP250, SSeCKS
A kinase (PRKA) anchor protein 12
GO Process (3)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ARFGEF1
ARFGEP1, BIG1, P200
ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
GO Process (10)
GO Function (6)
GO Component (9)
Gene Ontology Biological Process
- Golgi organization [IMP]
- endomembrane system organization [IMP]
- exocytosis [TAS]
- negative regulation of Rho GTPase activity [IDA]
- negative regulation of actin filament polymerization [IMP]
- positive regulation of GTPase activity [IBA, IDA]
- positive regulation of protein glycosylation in Golgi [IMP]
- positive regulation of wound healing [IMP]
- regulation of establishment of cell polarity [IMP]
- vesicle-mediated transport [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF7 cell line (score 0.661)
Curated By
- BioGRID