BAIT
CRTC1
MECT1, TORC-1, TORC1, WAMTP1
CREB regulated transcription coactivator 1
GO Process (3)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
WAC
BM-016, PRO1741, Wwp4, RP11-48B24.1
WW domain containing adaptor with coiled-coil
GO Process (7)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- G1 DNA damage checkpoint [IMP]
- cellular response to DNA damage stimulus [IMP]
- histone H2B conserved C-terminal lysine ubiquitination [IMP]
- histone monoubiquitination [IMP]
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- positive regulation of macroautophagy [IMP]
- positive regulation of transcription, DNA-templated [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleoplasm [IDA]
- nucleus [TAS]
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF7 cell line (score 0.678)
Curated By
- BioGRID