BAIT
CUL5
VACM-1, VACM1
cullin 5
GO Process (7)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ERO1L
ERO1-alpha, ERO1A, ERO1LA, UNQ434/PRO865
ERO1-like (S. cerevisiae)
GO Process (8)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- cellular protein metabolic process [TAS]
- cellular protein modification process [TAS]
- chaperone mediated protein folding requiring cofactor [IDA]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISS]
- protein folding [TAS]
- release of sequestered calcium ion into cytosol [ISS]
- response to endoplasmic reticulum stress [ISS]
- response to temperature stimulus [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.885)
- MCF7 cell line (score 0.726)
Curated By
- BioGRID