BAIT
AP1M1
AP47, CLAPM2, CLTNM, MU-1A
adaptor-related protein complex 1, mu 1 subunit
GO Process (7)
GO Function (1)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GOLPH3
GOPP1, GPP34, MIDAS, Vps74
golgi phosphoprotein 3 (coat-protein)
GO Process (15)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- Golgi organization [IMP]
- Golgi to plasma membrane protein transport [IMP]
- Golgi vesicle budding [IMP]
- cell adhesion molecule production [IMP]
- cell migration [IMP]
- cell proliferation [IMP]
- gene expression [IMP]
- glycoprotein biosynthetic process [IMP]
- lamellipodium assembly [IMP]
- leukocyte tethering or rolling [IMP]
- positive regulation of TOR signaling [IMP]
- positive regulation of protein secretion [IMP]
- protein retention in Golgi apparatus [IMP]
- protein secretion [IMP]
- regulation of mitochondrion organization [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF7 cell line (score 0.818)
Curated By
- BioGRID