BAIT
CHMP4A
C14orf123, CHMP4, CHMP4B, HSPC134, SHAX2, SNF7, SNF7-1, VPS32-1, VPS32A, CDA04
charged multivesicular body protein 4A
GO Process (11)
GO Function (4)
GO Component (7)
Gene Ontology Biological Process
- endosomal transport [TAS]
- membrane budding [IGI, IMP]
- membrane organization [TAS]
- membrane tubulation [IGI, IMP]
- negative regulation of autophagic vacuole assembly [IMP]
- negative regulation of neuron death [IMP]
- posttranslational protein targeting to membrane [IMP]
- protein homooligomerization [IMP]
- viral budding via host ESCRT complex [IGI]
- viral life cycle [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CHMP2B
ALS17, CHMP2.5, DMT1, VPS2-2, VPS2B, CGI-84
charged multivesicular body protein 2B
GO Process (5)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.75)
- MCF7 cell line (score 0.701)
- MDA231 cell line (score 0.735)
Curated By
- BioGRID