BAIT

ASAP1

AMAP1, CENTB4, DDEF1, PAG2, PAP, ZG14P
ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
GO Process (1)
GO Function (1)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

EXOC6B

SEC15B, SEC15L2
exocyst complex component 6B
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.

Havugimana PC, Goel RK, Phanse S, Youssef A, Padhorny D, Kotelnikov S, Kozakov D, Emili A

Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]

Nat Commun Jul. 13, 2022; 13(1);4043 [Pubmed: 35831314]

Throughput

  • High Throughput

Additional Notes

  • High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
  • MDA231 cell line (score 0.7)

Curated By

  • BioGRID