Publication

AKIRIN2 controls the nuclear import of proteasomes in vertebrates.

de Almeida M, Hinterndorfer M, Brunner H, Grishkovskaya I, Singh K, Schleiffer A, Jude J, Deswal S, Kalis R, Vunjak M, Lendl T, Imre R, Roitinger E, Neumann T, Kandolf S, Schutzbier M, Mechtler K, Versteeg GA, Haselbach D, Zuber J

Protein expression and turnover are controlled through a complex interplay of transcriptional, post-transcriptional and post-translational mechanisms to enable spatial and temporal regulation of cellular processes. To systematically elucidate such gene regulatory networks, we developed a CRISPR screening assay based on time-controlled Cas9 mutagenesis, intracellular immunostaining and fluorescence-activated cell sorting that enables the identification of regulatory factors independent of their effects ... [more]

Throughput

• High Throughput

Additional Notes

• Co-immunoprecipitation mass spectrometry (co-IP/MS) carried out using V5-tagged bait proteins expressed in RKO cells.
• Log2 Fold-change >2
• Adj. P-val <0.05

Curated By

• BioGRID