An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Prostate secretory protein 94 inhibits sterol binding and export by the mammalian CAP protein CRISP2 in a calcium-sensitive manner.

El Atab O, Kocabey AE, Asojo OA, Schneiter R

Members of the CAP protein superfamily are present in all kingdoms of life and have been implicated in many different processes, including pathogen defense, immune evasion, sperm maturation, and cancer progression. Most CAP proteins are secreted glycoproteins and share a unique conserved ??? sandwich fold. The precise mode of action of this class of proteins, however, has remained elusive. Saccharomyces ... [more]

J Biol Chem Dec. 01, 2021; 298(3);101600 [Pubmed: 35063506]

Throughput

Low Throughput

Additional Notes

Figure 2

Curated By

BioGRID

Interaction Summary

CRISP3

Gene Ontology Biological Process

Gene Ontology Cellular Component