Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

HERC5/IFI16/p53 signaling mediates breast cancer cell proliferation and migration.

Liu C, Li L, Hou G, Lu Y, Gao M, Zhang L

This study aims to find differentially expressed ubiquitination-related gene(s) and elucidates their biological significance in breast cancer.Differentially expressed genes were profiled in MCF-7 and MDA-MB-231 cells by using PCR array method. Abnormal expression of HERC5 was studied in the cells and in breast cancer specimens via Quantitative Real-time PCR and western blot. Cell proliferation and cell migration abilities were evaluated ... [more]

Life Sci Aug. 15, 2022; 303();120692 [Pubmed: 35671810]

Throughput

  • Low Throughput

Curated By

  • BioGRID