Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

MEX3A promotes nasopharyngeal carcinoma progression via the miR-3163/SCIN axis by regulating NF-?B signaling pathway.

Xiang XX, Liu YL, Kang YF, Lu X, Xu K

Mex-3 RNA Binding Family Member A (MEX3A) is an RNA-binding protein that plays complex and diverse roles in the development of various malignancies. However, its role and mechanism in nasopharyngeal carcinoma (NPC) remain undefined and were therefore evaluated in this study. By analyzing Gene Expression Omnibus data and using tissue microarrays, we found that MEX3A is significantly upregulated in NPC ... [more]

Cell Death Dis Apr. 30, 2022; 13(4);420 [Pubmed: 35490173]

Throughput

  • Low Throughput

Curated By

  • BioGRID