BAIT

BHLHE40

Bhlhb2, Dec1, SHARP-2, Sharp2, Stra13, Stra14
basic helix-loop-helix family, member e40
GO Process (11)
GO Function (13)
GO Component (4)

Gene Ontology Cellular Component

Rattus norvegicus
PREY

SEC31A

Sec31l1, VAP1
SEC31 homolog A (S. cerevisiae)
Rattus norvegicus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

DEC1 represses cardiomyocyte hypertrophy by recruiting PRP19 as an E3 ligase to promote ubiquitination-proteasome-mediated degradation of GATA4.

Cheng L, Xu C, Chen Y, Li J, Wang J, Xiao J, Ma M, Feng X, Wei X, Fang J

Although the pro-hypertrophic role of GATA binding protein 4 (GATA4) during cardiac hypertrophy has been well established, the negative regulatory mechanism to counteract its hyperactivation remains elusive. We hypothesized that the hyperactivation of GATA4 could be a result of loss of interaction between GATA4 with specific suppressors. Using high throughput mass spectrometry technology, we carried out a proteomic screen for ... [more]

J Mol Cell Cardiol Aug. 01, 2022; 169();96-110 [Pubmed: 35659652]

Throughput

  • High Throughput

Curated By

  • BioGRID