BAIT

BHLHE40

BHLHB2, DEC1, HLHB2, SHARP-2, STRA13, Stra14
basic helix-loop-helix family, member e40
Homo sapiens
PREY

CORO2B

CLIPINC
coronin, actin binding protein, 2B
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

DEC1 represses cardiomyocyte hypertrophy by recruiting PRP19 as an E3 ligase to promote ubiquitination-proteasome-mediated degradation of GATA4.

Cheng L, Xu C, Chen Y, Li J, Wang J, Xiao J, Ma M, Feng X, Wei X, Fang J

Although the pro-hypertrophic role of GATA binding protein 4 (GATA4) during cardiac hypertrophy has been well established, the negative regulatory mechanism to counteract its hyperactivation remains elusive. We hypothesized that the hyperactivation of GATA4 could be a result of loss of interaction between GATA4 with specific suppressors. Using high throughput mass spectrometry technology, we carried out a proteomic screen for ... [more]

J Mol Cell Cardiol Aug. 01, 2022; 169();96-110 [Pubmed: 35659652]

Throughput

  • Low Throughput

Curated By

  • BioGRID