BAIT

URB2

NPA2, YJR041C
Protein required for normal metabolism of the rRNA primary transcript; nucleolar protein; proposed to be involved in ribosome biogenesis
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

NOP1

LOT3, rRNA methyltransferase NOP1, L000001260, YDL014W
Histone glutamine methyltransferase, modifies H2A at Q105 in nucleolus; component of the small subunit processome complex, which is required for processing of pre-18S rRNA; ortholog of mammalian fibrillarin
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Emergence of the primordial pre-60S from the 90S pre-ribosome.

Ismail S, Flemming D, Thoms M, Gomes-Filho JV, Randau L, Beckmann R, Hurt E

Synthesis of ribosomes begins in the nucleolus with formation of the 90S pre-ribosome, during which the pre-40S and pre-60S pathways diverge by pre-rRNA cleavage. However, it remains unclear how, after this uncoupling, the earliest pre-60S subunit continues to develop. Here, we reveal a large-subunit intermediate at the beginning of its construction when still linked to the 90S, the precursor to ... [more]

Cell Rep Dec. 05, 2021; 39(1);110640 [Pubmed: 35385737]

Throughput

  • High Throughput

Additional Notes

  • Sequential AC, Urb2 and Noc1
  • Sequential AC, Urb2 and Nop58
  • Sequential AC, Urb2 and Nsa3

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
URB2 NOP1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.184BioGRID
1940035

Curated By

  • BioGRID