BAIT

PARK2

AR-JP, LPRS2, PDJ, PRKN, KB-152G3.1
parkin RBR E3 ubiquitin protein ligase
GO Process (58)
GO Function (22)
GO Component (12)

Gene Ontology Biological Process

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling.

Zhai Y, Huang X, Zhang K, Huang Y, Jiang Y, Cui J, Zhang Z, Chiu CKC, Zhong W, Li G

Enzymatic-based proximity labeling approaches based on activated esters or phenoxy radicals have been widely used for mapping subcellular proteome and protein interactors in living cells. However, activated esters are poorly reactive which leads to a wide labeling radius and phenoxy radicals generated by peroxide treatment may disturb redox-sensitive pathways. Herein, we report a photoactivation-dependent proximity labeling (PDPL) method designed by ... [more]

Nat Commun Aug. 20, 2022; 13(1);4906 [Pubmed: 35987950]

Throughput

  • High Throughput

Additional Notes

  • incorporation of miniSOG at the C termini of Parkin
  • incorporation of miniSOG at the N termini of Parkin
  • photoactivation-dependent proximity labeling (PDPL)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DUT PARK2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
3458725

Curated By

  • BioGRID