TRIM27
Gene Ontology Biological Process
- Arp2/3 complex-mediated actin nucleation [IDA]
- cell proliferation [TAS]
- innate immune response [IDA]
- interferon-gamma secretion [IMP]
- negative regulation of adaptive immune response [IMP]
- negative regulation of calcium ion import [IMP]
- negative regulation of gene expression, epigenetic [IDA]
- negative regulation of interleukin-2 secretion [IMP]
- negative regulation of protein kinase activity [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of tumor necrosis factor production [IMP]
- negative regulation of viral transcription [IDA]
- peptidyl-tyrosine phosphorylation [TAS]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- protein K63-linked ubiquitination [IMP]
- protein trimerization [IDA]
- retrograde transport, endosome to Golgi [IDA]
- spermatogenesis [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UBE2D3
Gene Ontology Biological Process
- BMP signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- cellular protein modification process [TAS]
- cellular response to hypoxia [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of type I interferon production [TAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein K11-linked ubiquitination [IDA]
- protein K48-linked ubiquitination [IDA]
- protein monoubiquitination [IDA]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
TRIM27 cooperates with STK38L to inhibit ULK1-mediated autophagy and promote tumorigenesis.
Autophagy represents a fundamental mechanism for maintaining cell survival and tissue homeostasis in response to physiological and?pathological stress. Autophagy initiation converges on the FIP200-ATG13-ULK1 complex wherein the serine/threonine kinase ULK1 plays a central role. Here, we reveal that the E3 ubiquitin ligase?TRIM27 functions as a negative regulatory component of the FIP200-ATG13-ULK1 complex. TRIM27 directly polyubiquitinates ULK1 at K568 and K571 ... [more]
Throughput
- Low Throughput
Additional Notes
- The reaction contained UBA1 [E1], UBE2D3 [E2], and TRIM27 [E3]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBE2D3 TRIM27 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3872405 | |
| TRIM27 UBE2D3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| UBE2D3 TRIM27 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | 5 | BioGRID | - | |
| TRIM27 UBE2D3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| UBE2D3 TRIM27 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID