An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Dietary folate drives methionine metabolism to promote cancer development by stabilizing MAT IIA.

Li JT, Yang H, Lei MZ, Zhu WP, Su Y, Li KY, Zhu WY, Wang J, Zhang L, Qu J, Lv L, Lu HJ, Chen ZJ, Wang L, Yin M, Lei QY

Folic acid, served as dietary supplement, is closely linked to one-carbon metabolism and methionine metabolism. Previous clinical evidence indicated that folic acid supplementation displays dual effect on cancer development, promoting or suppressing tumor formation and progression. However, the underlying mechanism remains to be uncovered. Here, we report that high-folate diet significantly promotes cancer development in mice with hepatocellular carcinoma (HCC) ... [more]

Signal Transduct Target Ther Dec. 22, 2021; 7(1);192 [Pubmed: 35729157]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

VCPIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin-specific protease activity [IDA, ISS]

Gene Ontology Cellular Component

MAT2A