An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Elucidation of the BMI1 interactome identifies novel regulatory roles in glioblastoma.

Freire-Beneitez V, Pomella N, Millner TO, Dumas AA, Niklison-Chirou MV, Maniati E, Wang J, Rajeeve V, Cutillas P, Marino S

Glioblastoma (GBM) is the most common and aggressive intrinsic brain tumour in adults. Epigenetic mechanisms controlling normal brain development are often dysregulated in GBM. Among these, BMI1, a structural component of the Polycomb Repressive Complex 1 (PRC1), which promotes the H2AK119ub catalytic activity of Ring1B, is upregulated in GBM and its tumorigenic role has been shown in vitro and in ... [more]

NAR Cancer Mar. 01, 2021; 3(1);zcab009 [Pubmed: 34316702]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BMI1 CUL4A	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Han K (2017)	High	-2.092	BioGRID	2538932

Curated By

BioGRID

Interaction Summary

BMI1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RING-like zinc finger domain binding [IPI]protein binding [IPI]zinc ion binding [IDA]

Gene Ontology Cellular Component

CUL4A