Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

TRIM27 cooperates with STK38L to inhibit ULK1-mediated autophagy and promote tumorigenesis.

Yang Y, Zhu Y, Zhou S, Tang P, Xu R, Zhang Y, Wei D, Wen J, Thorne RF, Zhang XD, Guan JL, Liu L, Wu M, Chen S

Autophagy represents a fundamental mechanism for maintaining cell survival and tissue homeostasis in response to physiological and?pathological stress. Autophagy initiation converges on the FIP200-ATG13-ULK1 complex wherein the serine/threonine kinase ULK1 plays a central role. Here, we reveal that the E3 ubiquitin ligase?TRIM27 functions as a negative regulatory component of the FIP200-ATG13-ULK1 complex. TRIM27 directly polyubiquitinates ULK1 at K568 and K571 ... [more]

EMBO J Dec. 18, 2021; 41(14);e109777 [Pubmed: 35670107]

Throughput

  • Low Throughput

Curated By

  • BioGRID