An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Renal UTX-PHGDH-serine axis regulates metabolic disorders in the kidney and liver.

Chen H, Liu C, Wang Q, Xiong M, Zeng X, Yang D, Xie Y, Su H, Zhang Y, Huang Y, Chen Y, Yue J, Liu C, Wang S, Huang K, Zheng L

Global obesity epidemics impacts human health and causes obesity-related illnesses, including the obesity-related kidney and liver diseases. UTX, a histone H3K27 demethylase, plays important roles in development and differentiation. Here we show that kidney-specific knockout Utx inhibits high-fat diet induced lipid accumulation in the kidney and liver via upregulating circulating serine levels. Mechanistically, UTX recruits E3 ligase RNF114 to ubiquitinate ... [more]

Nat Commun Dec. 04, 2021; 13(1);3835 [Pubmed: 35788583]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PHGDH

Gene Ontology Biological Process

Gene Ontology Molecular Function

electron carrier activity [TAS]phosphoglycerate dehydrogenase activity [TAS]

Gene Ontology Cellular Component

KDM6A