Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Neuronal-specific septin-3 binds Atg8/LC3B, accumulates and localizes to autophagosomes during induced autophagy.

Toth V, Vadaszi H, Ravasz L, Mittli D, Matyas D, Molnar T, Micsonai A, Szaniszlo T, Lorincz P, Kovacs R A, Juhasz T, Beke-Somfai T, Juhasz G, Gyoerffy BA, Kekesi KA, Kardos J

In synapses that show signs of local apoptosis and mitochondrial stress and undergo neuro-immunological synapse pruning, an increase in the levels of the presynaptic protein, neuronal-specific septin-3 can be observed. Septin-3 is a member of the septin GTPase family with the ability to form multimers and contribute to the cytoskeleton. However, the function of septin-3 remains elusive. Here, we provide ... [more]

Cell Mol Life Sci Aug. 06, 2022; 79(9);471 [Pubmed: 35932293]

Throughput

  • Low Throughput

Additional Notes

  • Used commercial HIS-tagged interactor A and B

Curated By

  • BioGRID