Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

EVI1 oncoprotein interacts with a large and complex network of proteins and integrates signals through protein phosphorylation.

Bard-Chapeau EA, Gunaratne J, Kumar P, Chua BQ, Muller J, Bard FA, Blackstock W, Copeland NG, Jenkins NA

Ecotropic viral integration site-1 (EVI1) is an oncogenic zinc finger transcription factor whose expression is frequently up-regulated in myeloid leukemia and epithelial cancers. To better understand the mechanisms underlying EVI1-associated disease, we sought to define the EVI1 interactome in cancer cells. By using stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics, we could confidently assign 78 ... [more]

Proc Natl Acad Sci U S A Jul. 30, 2013; 110(31);E2885-94 [Pubmed: 23858473]

Throughput

  • High Throughput

Curated By

  • BioGRID