MECOM
Gene Ontology Biological Process
- hematopoietic stem cell proliferation [ISS]
- negative regulation of JNK cascade [IMP]
- negative regulation of programmed cell death [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of cell cycle [IDA]
- regulation of transcription, DNA-templated [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TJP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
EVI1 oncoprotein interacts with a large and complex network of proteins and integrates signals through protein phosphorylation.
Ecotropic viral integration site-1 (EVI1) is an oncogenic zinc finger transcription factor whose expression is frequently up-regulated in myeloid leukemia and epithelial cancers. To better understand the mechanisms underlying EVI1-associated disease, we sought to define the EVI1 interactome in cancer cells. By using stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics, we could confidently assign 78 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MECOM TJP1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID