BTRC
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IBA]
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- mitotic cell cycle [TAS]
- negative regulation of sequence-specific DNA binding transcription factor activity [TAS]
- negative regulation of smoothened signaling pathway [TAS]
- negative regulation of transcription, DNA-templated [IMP]
- positive regulation of circadian rhythm [ISS]
- positive regulation of proteolysis [IMP]
- positive regulation of transcription, DNA-templated [ISS]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein dephosphorylation [ISS]
- protein destabilization [IMP]
- protein ubiquitination [IDA]
- regulation of circadian rhythm [IDA]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- signal transduction [TAS]
- ubiquitin-dependent protein catabolic process [IDA]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NFE2L2
Gene Ontology Biological Process
- cellular response to fluid shear stress [IDA]
- cellular response to hydrogen peroxide [IMP]
- cellular response to laminar fluid shear stress [IMP]
- cellular response to tumor necrosis factor [IMP]
- negative regulation of endothelial cell apoptotic process [IMP]
- negative regulation of hydrogen peroxide-induced cell death [IGI]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [IMP]
- positive regulation of gene expression [IGI]
- positive regulation of transcription from RNA polymerase II promoter [IC, IDA, IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to stress [IMP]
- proteasomal ubiquitin-independent protein catabolic process [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein ubiquitination [IDA]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [TAS]
- transcription regulatory region sequence-specific DNA binding [TAS]
- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [TAS]
- transcription regulatory region sequence-specific DNA binding [TAS]
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
An inhibitor of interaction between the transcription factor NRF2 and the E3 ubiquitin ligase adapter ?-TrCP delivers anti-inflammatory responses in mouse liver.
It is widely accepted that activating the transcription factor NRF2 will blast the physiological anti-inflammatory mechanisms, which will help combat pathologic inflammation. Much effort is being put in inhibiting the main NRF2 repressor, KEAP1, with either electrophilic small molecules or disrupters of the KEAP1/NRF2 interaction. However, targeting ?-TrCP, the non-canonical repressor of NRF2, has not been considered yet. After in ... [more]
Throughput
- Low Throughput
Additional Notes
- E2:UBE2R2
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BTRC NFE2L2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1239145 | |
| BTRC NFE2L2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NFE2L2 BTRC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NFE2L2 BTRC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| BTRC NFE2L2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| BTRC NFE2L2 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | 3462654 | |
| NFE2L2 BTRC | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | Low | - | BioGRID | 3012613 | |
| BTRC NFE2L2 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 1425685 | |
| NFE2L2 BTRC | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 3403588 | |
| BTRC NFE2L2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 3403592 |
Curated By
- BioGRID