BAIT

S

spike, SARS-CoV2 S, SARS-CoV2 spike, S protein, surface, SARS-CoV-2 spike, SARS-CoV-2 S, SPIKE_SARS2, PRO_0000449646, GU280_gp02
Spike glycoprotein; Surface glycoprotein
COVID-19 Coronavirus Project
Synthetic Protein Interaction Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

IFITM2

1-8D, DSPA2c
interferon induced transmembrane protein 2
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro.

Prelli Bozzo C, Nchioua R, Volcic M, Koepke L, Krueger J, Schuetz D, Heller S, Stuerzel CM, Kmiec D, Conzelmann C, Mueller J, Zech F, Braun E, Gross R, Wettstein L, Weil T, Weiss J, Diofano F, Rodriguez Alfonso AA, Wiese S, Sauter D, Muench J, Goffinet C, Catanese A, Schoen M, Boeckers TM, Stenger S, Sato K, Just S, Kleger A, Sparrer KMJ, Kirchhoff F

Interferon-induced transmembrane proteins (IFITMs 1, 2 and 3) can restrict viral pathogens, but pro- and anti-viral activities have been reported for coronaviruses. Here, we show that artificial overexpression of IFITMs blocks SARS-CoV-2 infection. However, endogenous IFITM expression supports efficient infection of SARS-CoV-2 in human lung cells. Our results indicate that the SARS-CoV-2 Spike protein interacts with IFITMs and hijacks them ... [more]

Nat Commun Dec. 28, 2020; 12(1);4584 [Pubmed: 34321474]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
S IFITM2
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
3458009
S IFITM2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
3458006

Curated By

  • BioGRID