Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Functional skewing of TRIM21-SIRT5 interplay dictates IL-1? production in DSS-induced colitis.

Yao P, Chen T, Jiang P, Li L, Du W

Macrophage polarization determines the production of pro- or anti-inflammatory cytokines in response to various bacterial and virus infections. Here, we report that pro-inflammatory macrophage polarization induced by lipopolysaccharide (LPS) skews the TRIM21-SIRT5 interplay toward TRIM21 activation and SIRT5 degradation, resulting in an enhancement of interleukin (IL)-1? production in?vitro and in?vivo. Mechanistically, LPS challenge enhances the interaction between TRIM21 and SIRT5 ... [more]

EMBO Rep Dec. 05, 2021; 23(9);e54391 [Pubmed: 35770730]

Throughput

  • Low Throughput

Curated By

  • BioGRID