An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins.

May DG, Martin-Sancho L, Anschau V, Liu S, Chrisopulos RJ, Scott KL, Halfmann CT, Diaz Pena R, Pratt D, Campos AR, Roux KJ

The novel coronavirus SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic and has caused a major health and economic burden worldwide. Understanding how SARS-CoV-2 viral proteins behave in host cells can reveal underlying mechanisms of pathogenesis and assist in development of antiviral therapies. Here, the cellular impact of expressing SARS-CoV-2 viral proteins was studied by global proteomic analysis, and proximity ... [more]

Viruses Dec. 15, 2021; 14(3); [Pubmed: 35337019]

Throughput

High Throughput

Additional Notes

BioID system
Significant viral-host BioID protein interaction with a log2FC >= 2.3, p value =< 0.01, at least two quantitative peptide features, and detected in less than 50% of the proximity-labeling CRAPome contaminant database experiments.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NSP4 LCLAT1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Samavarchi-Tehrani P (2020)	High	14.83	BioGRID	2792257

Curated By

BioGRID

Interaction Summary

NSP4

LCLAT1

Gene Ontology Biological Process

Gene Ontology Cellular Component