BAIT
BAP1
HUCEP-13, UCHL2, hucep-6
BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
GO Process (8)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
- cellular protein modification process [NAS]
- monoubiquitinated histone H2A deubiquitination [IDA]
- monoubiquitinated protein deubiquitination [IDA]
- negative regulation of cell proliferation [TAS]
- protein K48-linked deubiquitination [IMP]
- protein deubiquitination [IDA]
- regulation of cell cycle [IMP]
- regulation of cell growth [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
HSP90B1
ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1
heat shock protein 90kDa beta (Grp94), member 1
GO Process (13)
GO Function (6)
GO Component (13)
Gene Ontology Biological Process
- ER-associated ubiquitin-dependent protein catabolic process [IMP]
- actin rod assembly [IDA]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- cellular protein metabolic process [TAS]
- cellular response to ATP [IDA]
- endoplasmic reticulum unfolded protein response [TAS]
- innate immune response [TAS]
- negative regulation of apoptotic process [IMP, TAS]
- protein transport [NAS]
- regulation of phosphoprotein phosphatase activity [IDA]
- response to hypoxia [IDA]
- sequestering of calcium ion [NAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [IDA]
- endocytic vesicle lumen [TAS]
- endoplasmic reticulum [IDA, TAS]
- endoplasmic reticulum lumen [IDA, TAS]
- endoplasmic reticulum membrane [IDA]
- extracellular matrix [IDA]
- extracellular region [TAS]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- midbody [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Tumor suppressor BAP1 nuclear import is governed by transportin-1.
Subcellular localization of the deubiquitinating enzyme BAP1 is deterministic for its tumor suppressor activity. While the monoubiquitination of BAP1 by an atypical E2/E3-conjugated enzyme UBE2O and BAP1 auto-deubiquitination are known to regulate its nuclear localization, the molecular mechanism by which BAP1 is imported into the nucleus has remained elusive. Here, we demonstrated that transportin-1 (TNPO1, also known as Karyopherin ?2 ... [more]
J Cell Biol Dec. 06, 2021; 221(6); [Pubmed: 35446349]
Throughput
- High Throughput
Additional Notes
- Affinity capture MS was carried out to identify high confidence protein-protein interactors.
Curated By
- BioGRID