Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Tumor suppressor BAP1 nuclear import is governed by transportin-1.

Yang TJ, Li TN, Huang RS, Pan MY, Lin SY, Lin S, Wu KP, Wang LH, Hsu SD

Subcellular localization of the deubiquitinating enzyme BAP1 is deterministic for its tumor suppressor activity. While the monoubiquitination of BAP1 by an atypical E2/E3-conjugated enzyme UBE2O and BAP1 auto-deubiquitination are known to regulate its nuclear localization, the molecular mechanism by which BAP1 is imported into the nucleus has remained elusive. Here, we demonstrated that transportin-1 (TNPO1, also known as Karyopherin ?2 ... [more]

J Cell Biol Dec. 06, 2021; 221(6); [Pubmed: 35446349]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture MS was carried out to identify high confidence protein-protein interactors.

Curated By

  • BioGRID