BAIT

PLEKHA5

PEPP-2, PEPP2
pleckstrin homology domain containing, family A member 5
GO Process (0)
GO Function (2)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

PDZD11

AIPP1, PDZK11, PISP, RP13-26D14.3
PDZ domain containing 11
GO Process (0)
GO Function (2)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proximity Labeling Reveals Spatial Regulation of the Anaphase-Promoting Complex/Cyclosome by a Microtubule Adaptor.

Cao X, Shami Shah A, Sanford EJ, Smolka MB, Baskin JM

The anaphase-promoting complex/cyclosome (APC/C) coordinates advancement through mitosis via temporally controlled polyubiquitination events. Despite the long-appreciated spatial organization of key events in mitosis mediated largely by cytoskeletal networks, the spatial regulation of APC/C, the major mitotic E3 ligase, is poorly understood. We describe a microtubule-resident protein, PLEKHA5, as an interactor of APC/C and spatial regulator of its activity in mitosis. ... [more]

ACS Chem Biol Sep. 16, 2022; 17(9);2605-2618 [Pubmed: 35952650]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PLEKHA5 PDZD11
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2696685
PDZD11 PLEKHA5
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID