BAIT

OTUD1

DUBA7, OTDC1
OTU deubiquitinase 1
UPS Project
GO Process (1)
GO Function (1)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

PGAM5

BXLBV68
phosphoglycerate mutase family member 5
GO Process (3)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

OTUD1 deubiquitinase regulates NF-?B- and KEAP1-mediated inflammatory responses and reactive oxygen species-associated cell death pathways.

Oikawa D, Gi M, Kosako H, Shimizu K, Takahashi H, Shiota M, Hosomi S, Komakura K, Wanibuchi H, Tsuruta D, Sawasaki T, Tokunaga F

Deubiquitinating enzymes (DUBs) regulate numerous cellular functions by removing ubiquitin modifications. We examined the effects of 88 human DUBs on linear ubiquitin chain assembly complex (LUBAC)-induced NF-?B activation, and identified OTUD1 as a potent suppressor. OTUD1 regulates the canonical NF-?B pathway by hydrolyzing K63-linked ubiquitin chains from NF-?B signaling factors, including LUBAC. OTUD1 negatively regulates the canonical NF-?B activation, apoptosis, ... [more]

Cell Death Dis Aug. 08, 2022; 13(8);694 [Pubmed: 35941131]

Throughput

  • Low Throughput

Additional Notes

  • Interaction dependent on KEAP1

Curated By

  • BioGRID