BAIT

TRIM24

A130082H20Rik, AI447469, D430004I05Rik, TIF1, TIF1-alpha, TIF1alpha, Tif1a
tripartite motif-containing 24
Mus musculus
PREY

DDX6

1110001P04Rik, E230023J21Rik, HLR2, mRCK/P54, p54, rck
DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
GO Process (1)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

TRIM24 is an insulin-responsive regulator of P-bodies.

Wei W, Chen Q, Liu M, Sheng Y, OuYang Q, Feng W, Yang X, Ding L, Su S, Zhang J, Fang L, Vidal-Puig A, Wang HY, Chen S

Insulin is a potent inducer of mRNA transcription and translation, contributing to metabolic regulation. Insulin has also been suggested to regulate mRNA stability through the processing body (P-body) molecular machinery. However, whether and how insulin regulates mRNA stability via P-bodies is not clear. Here we show that the E3-ligase TRIM24 is a critical factor linking insulin signalling to P-bodies. Upon ... [more]

Nat Commun Dec. 08, 2021; 13(1);3972 [Pubmed: 35803934]

Throughput

  • Low Throughput

Curated By

  • BioGRID