An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Spike S1 domain interactome in non-pulmonary systems: A role beyond the receptor recognition.

Iacobucci I, Monaco V, Cane L, Bibbo F, Cioffi V, Cozzolino F, Guarino A, Zollo M, Monti M

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes Coronavirus Disease 2019 (COVID-19), which, since 2019 in China, has rapidly become a worldwide pandemic. The aggressiveness and global spread were enhanced by the many SARS-CoV-2 variants that have been isolated up to now. These mutations affect mostly the viral glycoprotein Spike (S), the capsid protein mainly involved in the early stages ... [more]

Front Mol Biosci Oct. 14, 2022; 9();975570 [Pubmed: 36225252]

Throughput

High Throughput

Additional Notes

HK-2 human kidney cell line
High confidence interaction with S1 subunit from SARS-CoV-2 Spike.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
S ATP5H	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Monaco V (2024)	High	-	BioGRID	3743878

Curated By

BioGRID

Interaction Summary

S

ATP5H

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [IDA]transmembrane transporter activity [IC]

Gene Ontology Cellular Component

Affinity Capture-MS