Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

TRABID targets DDB2 for deubiquitination to promote proliferation of hepatocellular carcinoma cells.

Chen Y, Zhang X

TRAF-binding domain-containing protein (TRABID), a member of the OTU deubiquitinase family, has an important role in regulating cellular functions via deubiquitinating substrate proteins such as EZH2 and Jmjd2d. However, the mechanism of its role in the proliferation of hepatocellular carcinoma (HCC) cells has not been fully elucidated. Here, we analyzed the interactome of TRABID in HepG2 cells through mass spectrometry-based ... [more]

Biochem Biophys Res Commun Dec. 15, 2021; 625();23-30 [Pubmed: 35944360]

Throughput

  • Low Throughput

Curated By

  • BioGRID