Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis.

McMahon KA, Wu Y, Gambin Y, Sierecki E, Tillu VA, Hall T, Martel N, Okano S, Moradi SV, Ruelcke JE, Ferguson C, Yap AS, Alexandrov K, Hill MM, Parton RG

Caveolae are specialized domains of the plasma membrane. Formation of these invaginations is dependent on the expression of Caveolin-1 or -3 and proteins of the cavin family. In response to stress, caveolae disassemble and cavins are released from caveolae, allowing cavins to potentially interact with intracellular targets. Here, we describe the intracellular (non-plasma membrane) cavin interactome using biotin affinity proteomics ... [more]

Nat Commun Dec. 22, 2018; 10(1);3279 [Pubmed: 31332168]

Throughput

  • Low Throughput

Curated By

  • BioGRID