An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A protein-RNA interaction atlas of the ribosome biogenesis factor AATF.

Kaiser RWJ, Ignarski M, Van Nostrand EL, Frese CK, Jain M, Cukoski S, Heinen H, Schaechter M, Seufert L, Bunte K, Frommolt P, Keller P, Helm M, Bohl K, Hoehne M, Schermer B, Benzing T, Hoepker K, Dieterich C, Yeo GW, Mueller RU, Fabretti F

AATF is a central regulator of the cellular outcome upon p53 activation, a finding that has primarily been attributed to its function as a transcription factor. Recent data showed that AATF is essential for ribosome biogenesis and plays a role in rRNA maturation. AATF has been implicated to fulfil this role through direct interaction with rRNA and was identified in ... [more]

Sci Rep Dec. 30, 2018; 9(1);11071 [Pubmed: 31363146]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AATF AATF	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Horlbeck MA (2018)	High	-4.6563	BioGRID	2455955

Curated By

BioGRID

Interaction Summary

AATF

Gene Ontology Biological Process

Gene Ontology Molecular Function

leucine zipper domain binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]sequence-specific DNA binding transcription factor activity [IDA]

Gene Ontology Cellular Component