Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A protein-RNA interaction atlas of the ribosome biogenesis factor AATF.

Kaiser RWJ, Ignarski M, Van Nostrand EL, Frese CK, Jain M, Cukoski S, Heinen H, Schaechter M, Seufert L, Bunte K, Frommolt P, Keller P, Helm M, Bohl K, Hoehne M, Schermer B, Benzing T, Hoepker K, Dieterich C, Yeo GW, Mueller RU, Fabretti F

AATF is a central regulator of the cellular outcome upon p53 activation, a finding that has primarily been attributed to its function as a transcription factor. Recent data showed that AATF is essential for ribosome biogenesis and plays a role in rRNA maturation. AATF has been implicated to fulfil this role through direct interaction with rRNA and was identified in ... [more]

Sci Rep Dec. 30, 2018; 9(1);11071 [Pubmed: 31363146]

Throughput

  • High Throughput

Curated By

  • BioGRID