BAIT

TM1

1305/10, 2299, BcDNA:GH09289, BcDNA:LD37158, BcDNA:SD21996, CG4898, Dm Tm1, Dm TmH33, Dm TmH34, DmTm1, Dmel\CG4898, PmI, TM, TMII, TmH, TmH-33, TmH-34, TmH33, TmH34, Tmr33, Tmr34, TnH, TnH-33, TnH-34, cTM, cTmII, chr3R:11122272..11122408, l(3)02299, l(3)S130510, l(3)s2958, mTmII, region 3, tropomyosin, Dmel_CG4898
Tropomyosin 1
Drosophila melanogaster
PREY

TM1

1305/10, 2299, BcDNA:GH09289, BcDNA:LD37158, BcDNA:SD21996, CG4898, Dm Tm1, Dm TmH33, Dm TmH34, DmTm1, Dmel\CG4898, PmI, TM, TMII, TmH, TmH-33, TmH-34, TmH33, TmH34, Tmr33, Tmr34, TnH, TnH-33, TnH-34, cTM, cTmII, chr3R:11122272..11122408, l(3)02299, l(3)S130510, l(3)s2958, mTmII, region 3, tropomyosin, Dmel_CG4898
Tropomyosin 1
Drosophila melanogaster

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A protein interaction map of Drosophila melanogaster.

Giot L, Bader JS, Brouwer C, Chaudhuri A, Kuang B, Li Y, Hao YL, Ooi CE, Godwin B, Vitols E, Vijayadamodar G, Pochart P, Machineni H, Welsh M, Kong Y, Zerhusen B, Malcolm R, Varrone Z, Collis A, Minto M, Burgess S, McDaniel L, Stimpson E, Spriggs F, Williams J, Neurath K, Ioime N, Agee M, Voss E, Furtak K, Renzulli R, Aanensen N, Carrolla S, Bickelhaupt E, Lazovatsky Y, DaSilva A, Zhong J, Stanyon CA, Finley RL, White KP, Braverman M, Jarvie T, Gold S, Leach M, Knight J, Shimkets RA, McKenna MP, Chant J, Rothberg JM

Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence ... [more]

Science Dec. 05, 2003; 302(5651);1727-36 [Pubmed: 14605208]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TM1 TM1
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Low-FlyBase
-
TM1 TM1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-FlyBase
-

Curated By

  • BioGRID