PNKP
Gene Ontology Biological Process
- DNA damage response, detection of DNA damage [IDA, NAS]
- DNA repair [IGI]
- DNA-dependent DNA replication [NAS]
- dephosphorylation [IDA]
- nucleic acid phosphodiester bond hydrolysis [NAS]
- nucleotide phosphorylation [IDA]
- nucleotide-excision repair, DNA damage removal [NAS]
- polynucleotide 3' dephosphorylation [IDA]
- response to oxidative stress [IDA]
- response to radiation [NAS, TAS]
Gene Ontology Molecular Function- ATP binding [NAS]
- ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity [IDA]
- damaged DNA binding [NAS]
- double-stranded DNA binding [TAS]
- endonuclease activity [NAS]
- nucleotide kinase activity [IDA]
- polynucleotide 3'-phosphatase activity [IDA]
- protein binding [IPI]
- purine nucleotide binding [NAS]
- ATP binding [NAS]
- ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity [IDA]
- damaged DNA binding [NAS]
- double-stranded DNA binding [TAS]
- endonuclease activity [NAS]
- nucleotide kinase activity [IDA]
- polynucleotide 3'-phosphatase activity [IDA]
- protein binding [IPI]
- purine nucleotide binding [NAS]
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
The Rev1 interacting region (RIR) motif in the scaffold protein XRCC1 mediates a low-affinity interaction with polynucleotide kinase/phosphatase (PNKP) during DNA single-strand break repair.
The scaffold protein X-ray repair cross-complementing 1 (XRCC1) interacts with multiple enzymes involved in DNA base excision repair and single-strand break repair (SSBR) and is important for genetic integrity and normal neurological function. One of the most important interactions of XRCC1 is that with polynucleotide kinase/phosphatase (PNKP), a dual-function DNA kinase/phosphatase that processes damaged DNA termini and that, if mutated, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| XRCC1 PNKP | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.431 | BioGRID | 241845 | |
| PNKP XRCC1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.504 | BioGRID | 241627 | |
| XRCC1 PNKP | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1451396 | |
| XRCC1 PNKP | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3268327 | |
| PNKP XRCC1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| XRCC1 PNKP | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| XRCC1 PNKP | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| XRCC1 PNKP | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PNKP XRCC1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PNKP XRCC1 | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | - | |
| XRCC1 PNKP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID