Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

PRDM14 directly interacts with heat shock proteins HSP90? and glucose-regulated protein 78.

Moriya C, Taniguchi H, Nagatoishi S, Igarashi H, Tsumoto K, Imai K

PRDM14 is overexpressed in various cancers and can regulate cancer phenotype under certain conditions. Inhibiting PRDM14 expression in breast and pancreatic cancers has been reported to reduce cancer stem-like phenotypes, which are associated with aggressive tumor properties. Therefore, PRDM14 is considered a promising target for cancer therapy. To develop a pharmaceutical treatment, the mechanism and interacting partners of PRDM14 need ... [more]

Cancer Sci Feb. 01, 2018; 109(2);373-383 [Pubmed: 29178343]

Throughput

  • High Throughput

Curated By

  • BioGRID