An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Modification of the base excision repair enzyme MBD4 by the small ubiquitin-like molecule SUMO1.

Sannai M, Doneddu V, Giri V, Seeholzer S, Nicolas E, Yip SC, Bassi MR, Mancuso P, Cortellino S, Cigliano A, Lurie R, Ding H, Chernoff J, Sobol RW, Yen TJ, Bagella L, Bellacosa A

The base excision repair DNA N-glycosylase MBD4 (also known as MED1), an interactor of the DNA mismatch repair protein MLH1, plays a central role in the maintenance of genomic stability of CpG sites by removing thymine and uracil from G:T and G:U mismatches, respectively. MBD4 is also involved in DNA damage response and transcriptional regulation. The interaction with other proteins ... [more]

DNA Repair (Amst) Dec. 01, 2018; 82();102687 [Pubmed: 31476572]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

MBD4

Gene Ontology Biological Process

Gene Ontology Molecular Function

endodeoxyribonuclease activity [TAS]protein binding [IPI]pyrimidine-specific mismatch base pair DNA N-glycosylase activity [IDA]satellite DNA binding [TAS]

Gene Ontology Cellular Component

HNRNPA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]single-stranded DNA binding [IDA]single-stranded RNA binding [IC]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Modification of the base excision repair enzyme MBD4 by the small ubiquitin-like molecule SUMO1.

Throughput

Curated By

endodeoxyribonuclease activity [TAS]
protein binding [IPI]
pyrimidine-specific mismatch base pair DNA N-glycosylase activity [IDA]
satellite DNA binding [TAS]

RNA binding [TAS]
poly(A) RNA binding [IDA]
protein binding [IPI]
single-stranded DNA binding [IDA]
single-stranded RNA binding [IC]