BAIT

GATA4

ASD2, TACHD, TOF, VSD1
GATA binding protein 4
GO Process (23)
GO Function (10)
GO Component (3)
Homo sapiens
PREY

EEF1D

EF-1D, EF1D, FP1047, UNQ601
eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Transcription factor protein interactomes reveal genetic determinants in heart disease.

Gonzalez-Teran B, Pittman M, Felix F, Thomas R, Richmond-Buccola D, Huettenhain R, Choudhary K, Moroni E, Costa MW, Huang Y, Padmanabhan A, Alexanian M, Lee CY, Maven BEJ, Samse-Knapp K, Morton SU, McGregor M, Gifford CA, Seidman JG, Seidman CE, Gelb BD, Colombo G, Conklin BR, Black BL, Bruneau BG, Krogan NJ, Pollard KS, Srivastava D

Congenital heart disease (CHD) is present in 1% of live births, yet identification of causal mutations remains challenging. We hypothesized that genetic determinants for CHDs may lie in the protein interactomes of transcription factors whose mutations cause CHDs. Defining the interactomes of two transcription factors haplo-insufficient in CHD, GATA4 and TBX5, within human cardiac progenitors, and integrating the results with ... [more]

Cell Dec. 03, 2021; 185(5);794-814.e30 [Pubmed: 35182466]

Throughput

  • High Throughput

Additional Notes

  • Experiment carried out in HEK293 cells

Curated By

  • BioGRID